Anim Biosci > Volume 35(4); 2022 > Article |
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Items | Control | F control | Lactobacillus Plantarum1) | Bacillus clausii1) | SEM | p-value | ||||||
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LP1 | LP2 | LP3 | LP4 | BC1 | BC2 | BC3 | BC4 | |||||
DM (fresh basis) | 51.77 | 47.53 | 50.09 | 51.06 | 51.65 | 50.69 | 47.17 | 46.53 | 48.51 | 50.19 | 0.499 | 0.107 |
CP | 13.61a | 14.64ab | 14.09ab | 14.29ab | 14.32ab | 14.23ab | 15.05b | 15.24b | 14.61ab | 14.63ab | 0.116 | 0.009 |
EE | 2.37 | 2.57 | 2.54 | 2.31 | 3.24 | 2.70 | 3.33 | 3.04 | 2.69 | 3.17 | 0.141 | 0.777 |
NDF | 38.17 | 35.34 | 35.14 | 33.34 | 34.47 | 34.92 | 35.74 | 37.48 | 35.57 | 37.32 | 0.401 | 0.162 |
ADF | 20.69a | 17.77ab | 16.67b | 16.34b | 15.38b | 15.99b | 16.67b | 17.72ab | 16.48b | 16.39b | 0.329 | 0.007 |
TMR, total mixed ration; CSM, cottonseed meal; DM, dry matter; SEM, pooled standard error of means; CP, crude protein; EE, ether extract; NDF, neutral detergent fiber; ADF, acid detergent fiber.
1) Treatments including control with no inoculant; F control, fermented control without inoculum; LP1, Lactobacillus plantarum with 1×108 CFU/kg DM; LP2, Lactobacillus plantarum with 1×109 CFU/kg DM; LP3, Lactobacillus plantarum with 1×1010 CFU/kg DM; LP4, Lactobacillus plantarum with 1×1011 CFU/kg DM; BC1, Bacillus clausii with 1×108 CFU/kg DM; BC2, Bacillus clausii with 1×109 CFU/kg DM; BC3, Bacillus clausii with 1×1010 CFU/kg DM; BC4, Bacillus clausii with 1×1011CFU/kg DM; CFU, colony-forming units.
Items | Control | F control | Saccharomyces cariocanus1) | Wickerhamomyces anomalus1) | SEM | p-value | ||||||
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SC1 | SC2 | SC3 | SC4 | WA1 | WA2 | WA3 | WA4 | |||||
DM (fresh basis) | 51.77 | 47.53 | 49.17 | 49.28 | 48.44 | 48.09 | 53.09 | 47.37 | 47.99 | 49.36 | 0.495 | 0.146 |
CP | 13.61 | 14.64 | 14.35 | 14.73 | 15.14 | 15.96 | 15.11 | 15.27 | 15.54 | 14.55 | 0.181 | 0.180 |
EE | 2.37 | 2.57 | 3.45 | 2.62 | 2.25 | 2.59 | 2.45 | 2.30 | 2.73 | 2.42 | 0.132 | 0.793 |
NDF | 38.17 | 35.34 | 34.02 | 32.95 | 34.21 | 34.30 | 35.35 | 34.43 | 34.62 | 33.02 | 0.405 | 0.192 |
ADF | 20.69 | 17.77 | 16.91 | 19.06 | 17.44 | 18.76 | 17.81 | 17.10 | 18.50 | 17.11 | 0.352 | 0.374 |
TMR, total mixed ration; CSM, cottonseed meal; DM, dry matter; SEM, pooled standard error of means; CP, crude protein; EE, ether extract; NDF, neutral detergent fiber; ADF, acid detergent fiber.
1) Treatments including control with no inoculant, F control, fermented without inoculum; SC1, Saccharomyces cariocanus with 5×106 CFU/kg DM; SC2, Saccharomyces cariocanus with 5×107 CFU/kg DM; SC3, Saccharomyces cariocanus with 5×108 CFU/kg DM SC4; Saccharomyces cariocanus with 5×109 CFU/kg DM; WA1, Wickerhamomyces anomalus with 5×106 CFU/kg DM; WA2, Wickerhamomyces anomalus with 5×107 CFU/kg DM; WA3, Wickerhamomyces anomalus with 5×108 CFU/kg DM; WA4, Wickerhamomyces anomalus with 5×109 CFU/kg DM; CFU, colony-forming units.
Items | Control | F control | Lactobacillus plantarum1) | Bacillus clausii1) | SEM | p-value | ||||||
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LP1 | LP2 | LP3 | LP4 | BC1 | BC2 | BC3 | BC4 | |||||
DM (fresh basis) | 52.72 | 49.90 | 49.72 | 48.84 | 49.61 | 50.61 | 49.31 | 49.32 | 48.34 | 49.70 | 0.344 | 0.289 |
CP | 12.68b | 13.33ab | 13.49ab | 13.62ab | 13.34ab | 13.23ab | 13.35ab | 13.31ab | 13.82a | 13.43ab | 0.071 | 0.045 |
EE | 1.91 | 2.39 | 1.95 | 1.97 | 2.00 | 1.54 | 2.31 | 2.09 | 2.08 | 1.975 | 0.100 | 0.902 |
NDF | 34.32b | 38.61ab | 37.26ab | 37.91ab | 37.83ab | 37.00ab | 38.29ab | 39.22a | 37.78ab | 39.25a | 0.342 | 0.043 |
ADF | 17.26 | 19.64 | 18.95 | 22.13 | 19.91 | 18.95 | 20.48 | 19.62 | 19.14 | 19.88 | 0.327 | 0.127 |
TMR, total mixed ration; RSM, rapeseed meal; DM, dry matter; SEM, pooled standard error of means; CP, crude protein; EE, ether extract; NDF, neutral detergent fiber; ADF, acid detergent fiber.
1) Treatments including control with no inoculant, F control, fermented without inoculum; LP1, Lactobacillus plantarum with 1×108 CFU/kg DM; LP2, Lactobacillus plantarum with 1×109 CFU/kg DM; LP3, Lactobacillus plantarum with 1×1010 CFU/kg DM; LP4, Lactobacillus plantarum with 1×1011 CFU/kg DM; BC1, Bacillus clausii with 1×108 CFU/kg DM; BC2, Bacillus clausii with 1×109 CFU/kg DM; BC3, Bacillus clausii with 1×1010 CFU/kg DM; BC4, Bacillus clausii with 1×1011 CFU/kg DM; CFU, colony-forming units.
Items | Control | F control | Saccharomyces cariocanus1) | Wickerhamomyces anomalus1) | SEM | p-value | ||||||
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SC1 | SC2 | SC3 | SC4 | WA1 | WA2 | WA3 | WA4 | |||||
DM (fresh basis) | 52.72 | 49.90 | 49.09 | 48.79 | 48.76 | 48.72 | 48.99 | 49.71 | 48.44 | 48.13 | 0.351 | 0.160 |
CP | 12.68b | 13.33ab | 13.46ab | 13.55ab | 13.66a | 13.94a | 13.59a | 13.62a | 13.69a | 14.11a | 0.082 | 0.002 |
EE | 1.91 | 2.39 | 1.77 | 2.08 | 2.16 | 1.94 | 2.31 | 1.50 | 2.01 | 2.52 | 0.089 | 0.306 |
NDF | 34.32b | 38.61ab | 36.57ab | 39.66a | 37.97ab | 37.38ab | 38.44ab | 38.89ab | 37.77ab | 39.82a | 0.385 | 0.033 |
ADF | 17.26 | 19.64 | 20.39 | 19.96 | 20.90 | 18.99 | 19.90 | 19.85 | 20.00 | 19.79 | 0.243 | 0.063 |
TMR, total mixed ration; RSM, rapeseed meal; DM, dry matter; SEM, pooled standard error of means; CP, crude protein; EE, ether extract; NDF, neutral detergent fiber; ADF, acid detergent fiber.
1) Treatments including control with no inoculant, F control, fermented control without inoculum; SC1, Saccharomyces cariocanus with 5×106 CFU/kg DM; SC2, Saccharomyces cariocanus with 5×107 CFU/kg DM; SC3, Saccharomyces cariocanus with 5×108 CFU/kg DM; SC4, Saccharomyces cariocanus with 5×109 CFU/kg DM; WA1, Wickerhamomyces anomalus with 5×106 CFU/kg DM; WA2, Wickerhamomyces anomalus with 5×107 CFU/kg DM; WA3, Wickerhamomyces anomalus with 5×108 CFU/kg DM; WA4, Wickerhamomyces anomalus with 5×109 CFU/kg DM; CFU, colony-forming units.
Items | Control | F control | Lactobacillus plantarum1) | Bacillus clasusii1) | SEM | p-value | ||||||
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LP1 | LP2 | LP3 | LP4 | BC1 | BC2 | BC3 | BC4 | |||||
CSM-based TMR | ||||||||||||
FG (μmol/g) | 92.94a | 77.18b | 73.71b | 75.84b | 75.13b | 68 87bc | 57.36c | 56.34c | 54.05c | 53.49c | 2.60 | <0.001 |
RSM-based TMR | ||||||||||||
GSLs (μmol/g) | 18.45a | 7.71b | 8.13b | 7.11b | 5.91bc | 6.20bc | 6.86b | 6.21bc | 5.86bc | 3.86c | 0.74 | <0.001 |
DM, dry matter; SEM, pooled standard error of means; CSM, cottonseed meal; TMR, total mixed ration; FG, free gossypol; RSM, rapeseed meal; GSLs, glucosinolates.
1) Treatments including control with no inoculant, F control, fermented control without inoculum; LP1, Lactobacillus plantarum with 1×108 CFU/kg DM; LP2, Lactobacillus plantarum with 1×109 CFU/kg DM; LP3, Lactobacillus plantarum with 1×1010 CFU/kg DM; LP4, Lactobacillus plantarum with 1×1011 CFU/kg DM; BC1, Bacillus clausii with 1×108 CFU/kg DM; BC2, Bacillus clausii with 1×109 CFU/kg DM; BC3, Bacillus clausii with 1×1010 CFU/kg DM; BC4, Bacillus clausii with 1×1011 CFU/kg DM; CFU, colony-forming units.
Items | Control | F Control | Saccharomyces cariocanus1) | Wickerhamomyces anomalus1) | SEM | p-value | ||||||
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SC1 | SC2 | SC3 | SC4 | WA1 | WA2 | WA3 | WA4 | |||||
CSM based TMR | ||||||||||||
FG (μmol/g) | 92.94c | 77.18bc | 57.82ab | 57.75ab | 54.17a | 52.23a | 56.59a | 50.64a | 61.21ab | 56.63a | 2.55 | <0.001 |
RSM-based TMR | ||||||||||||
GSLs (μmol/g) | 18.45a | 7.71b | 6.38b | 6.87b | 6.05b | 5.97b | 6.09b | 8.57b | 7.09b | 6.02b | 0.71 | <0.001 |
DM, dry matter; SEM, pooled standard error of means; CSM, cottonseed meal; TMR, total mixed ration; FG, free gossypol; RSM, rapeseed meal; GSLs, glucosinolates.
1) Treatments including control with no inoculant, F control, fermented control without inoculum; SC1, Saccharomyces cariocanus with 5×106 CFU/kg DM; SC2, Saccharomyces cariocanus with 5×107 CFU/kg DM; SC3, Saccharomyces cariocanus with 5×108 CFU/kg DM; SC4, Saccharomyces cariocanus with 5×109 CFU/kg DM; WA1, Wickerhamomyces anomalus with 5×106 CFU/kg DM; WA2, Wickerhamomyces anomalus with 5×107 CFU/kg DM; WA3, Wickerhamomyces anomalus with 5×108 CFU/kg DM; WA4, Wickerhamomyces anomalus with 5×109 CFU/kg DM; CFU, colony-forming units.